Acute high-altitude hypoxic brain injury Identification of ten differential proteins
Jianyu Li
Yuting Qi
Hui Liu
Ying Cui
Li Zhang
Haiying Gong
Yaxiao Li
Lingzhi Li
Yongliang Zhang
摘要：Hypobaric hypoxia can cause severe brain damage and mitochondrial dysfunction, and is involved in hypoxic brain injury. However, little is currently known about the mechanisms responsible for mi-tochondrial dysfunction in hypobaric hypoxic brain damage. In this study, a rat model of hypobaric hypoxic brain injury was established to investigate the molecular mechanisms associated with mi-tochondrial dysfunction. As revealed by two-dimensional electrophoresis analysis, 16, 21, and 36 differential protein spots in cerebral mitochondria were observed at 6, 12, and 24 hours post-hypobaric hypoxia, respectively. Furthermore, ten protein spots selected from each hypobaric hypoxia subgroup were similarly regulated and were identified by mass spectrometry. These de-tected proteins included dihydropyrimidinase-related protein 2, creatine kinase B-type, isova-leryl-CoA dehydrogenase, elongation factor Ts, ATP synthase beta-subunit, 3-mercaptopyruvate sulfurtransferase, electron transfer flavoprotein alpha-subunit, Chain A of 2-enoyl-CoA hydratase, NADH dehydrogenase iron-sulfur protein 8 and tropomyosin beta chain. These ten proteins are al involved in the electron transport chain and the function of ATP synthase. Our findings indicate that hypobaric hypoxia can induce the differential expression of several cerebral mitochondrial proteins, which are involved in the regulation of mitochondrial energy production.
机标关键词：differential proteinsIdentification ofmitochondrial dysfunctionelectrophoresis analysisbrain injurybrain damageATP synthasedifferential expression
资助基金：the National Natural Science Foundation of China, (No.81073152)the Key Science Foundation of Tianjin in China, (No.10JCZDJC21100)the Natural Science Foundation of Tianjin in China, (No.10JCYBJC14700, No.13JCQNJC13200)the Science Foundation of Tianjin Key Laboratory in China, (No. WHTD 201303-2)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 2932-2941 )
英文信息