Mannotriose regulates learning and memory signal transduction in the hippocampus
Lina Zhang
Weiwei Dai
Xueli Zhang
Zhangbin Gong
Guoqin Jin
摘要：Rehmannia is a commonly used Chinese herb, which improves learning and memory. However, the crucial components of the signal transduction pathway associated with this effect remain elusive. Pri-mary hippocampal neurons were cultured in vitro, insulted with high-concentration (1 × 10-4 mol/L) cor-ticosterone, and treated with 1 × 10-4 mol/L mannotriose. Thiazolyl blue tetrazolium bromide assay and western blot analysis showed that hippocampal neuron survival rates and protein levels of glucocorti-coid receptor, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor were al dramatical y decreased after high-concentration corticosterone-induced injury. This effect was reversed by mannotriose, to a similar level as RU38486 and donepezil. Our findings indicate that mannotriose could protect hippocampal neurons from high-concentration corticosterone-induced injury. The mechanism by which this occurred was associated with levels of glucocorticoid receptor protein, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor.
机标关键词：signal transduction pathwayneurotrophic factorhippocampal neuronsprotein kinaseglucocorticoid receptorwestern blot analysislearning and memorycultured in vitro
资助基金：This study was financial y supported by the National Natural Science Foundation of China, (No.30873317)the Leading Academic Discipline Project of Shanghai Municipal Education Commission, (No. J50301)“085 Class Disciplines Building”Scientific and Technology Innovation Fundation, (No.085ZY1204)Shanghai Health Bureau of Scientific Research Projects, (No.20124Y025)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 3020-3026 )
英文信息