Rho-associated protein kinase modulates neurite extension by regulating microtubule remodeling and vinculin distribution
Keen Chen
Wenbin Zhang
Jing Chen
Sumei Li
Guoqing Guo
摘要：Rho-associated protein kinase is an essential regulator of cytoskeletal dynamics during the process of neurite extension. However, whether Rho kinase regulates microtubule remodeling or the distri-bution of adhesive proteins to mediate neurite outgrowth remains unclear. By specifical y modulat-ing Rho kinase activity with pharmacological agents, we studied the morpho-dynamics of neurite outgrowth. We found that lysophosphatidic acid, an activator of Rho kinase, inhibited neurite out-growth, which could be reversed by Y-27632, an inhibitor of Rho kinase. Meanwhile, reorganization of microtubules was noticed during these processes, as indicated by their significant changes in the soma and growth cone. In addition, exposure to lysophosphatidic acid led to a decreased brane distribution of vinculin, a focal adhesion protein in neurons, whereas Y-27632 recruited culin to the membrane. Taken together, our data suggest that Rho kinase regulates rat hippocampal neurite growth and microtubule formation via a mechanism associated with the redistribution of vinculin.
机标关键词：Rho kinaselysophosphatidic acidneurite outgrowthprotein kinase
资助基金：the National Natural Science Foundation of China, (No.31170941)the Fundamental Research Funds for the Central Universities, (No.21612424)the Science and Technology Planning Project of Guangdong Province, (No.2010B031600102)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 3027-3035 )
英文信息