Targetingβ-secretase with RNAi in neural stem cells for Alzheimer’s disease therapy
Zhonghua Liu
Shengliang Li
Zibin Liang
Yan Zhao
Yulin Zhang
Yaqi Yang
Minjuan Wang
Feng Li
摘要：There are several major pathological changes in Alzheimer’s disease, including apoptosis of cho-linergic neurons, overactivity or overexpression ofβ-site amyloid precursor protein cleaving enzyme 1 (BACE1) and inflammation. In this study, we synthesized a 19-nt oligonucleotide targeting BACE1, the key enzyme in amyloid beta protein (Aβ) production, and introduced it into the pSilenCircle vector to construct a short hairpin (shRNA) expression plasmid against the BACE1 gene. We transfected this vector into C17.2 neural stem cells and primary neural stem cells, resulting in downregulation of the BACE1 gene, which in turn induced a considerable reduction in reducing Aβprotein production. We anticipate that this technique combining celltransplantation and gene ther-apy wil open up novel therapeutic avenues for Alzheimer’s disease, particularly because it can be used to simultaneously target several pathogenetic changes in the disease.
机标关键词：neural stem cellsamyloid precursor proteinamyloid beta proteinkey enzyme
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 3095-3106 )
英文信息