Phosphorylation of tau protein over time in rats subjected to transient brain ischemia
Bo Song
Qiang Ao
Zhen Wang
Weiqiang Liu
Ying Niu
Qin Shen
Huancong Zuo
Xiufang Zhang
Yandao Gong
摘要：Transient brain ischemia has been shown to induce hyperphosphorylation of the microtu-bule-associated protein tau. To further determine the mechanisms underlying these processes, we investigated the interaction between tau, glycogen synthase kinase (GSK)-3β and protein phos-phatase 2A. The results confirmed that tau protein was dephosphorylated during brain ischemia;in addition, the activity of GSK-3βwas increased and the activity of protein phosphatase 2A was de-creased. After reperfusion, tau protein was hyperphosphorylated, the activity of GSK-3β was de-creased and the activity of protein phosphatase 2A remained low. Importantly, the interaction of tau with GSK-3β and protein phosphatase 2A was altered during ischemia and reperfusion. Lithium chloride could affect tau phosphorylation by regulating the interaction of tau with GSK-3βand pro-tein phosphatase 2A, and improve learning and memory ability of rats after transient brain ischemia. The present study demonstrated that it was the interaction of tau with GSK-3β and protein phos-phatase 2A, rather than their individual activities, that dominates the phosphorylation of tau in tran-sient brain ischemia. Hyperphosphorylated tau protein may play an important role in the evolution of brain injury in ischemic stroke. The neuroprotective effects of lithium chloride partly depend on the inhibition of tau phosphorylation during transient brain ischemia.
机标关键词：brain ischemiaprotein phosphatasetau proteinlithium chlorideischemia and reperfusionneuroprotective effectslearning and memoryischemic stroke
资助基金：the Biological Industry Development Funds of Shenzhen, (No. JC201005260093A)the National Natural Science Foundation of China/Research Grants Council Joint Research Scheme, (No.81161160570)the National Natural Science Foundation of China, (No.81171143)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 3173-3182 )
英文信息