Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA Determination of an optimal transfection sequence
Guiyun Cui
Xiaopeng Wang
Xinchun Ye
Jie Zu
Kun Zan
Fang Hua
摘要：Tol-like receptor 3 protein expression has been shown to be upregulated during cerebral ische-mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy-gen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemical y synthesized smal interfering RNA (siRNA)-1280,-1724 and-418 specific to tol-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of tol-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, tol-like ceptor 3 protein expression reduced, especial y in the tol-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting tol-like receptor 3 expression in cortical neurons fol owing oxygen-glucose deprivation.
机标关键词：protein expressioncerebral ischemia
资助基金：This study was supported by the National Natural Science Foundation of China, (No.30970995)the Postgraduate Science Research Innovation Project of Higher Learning University of Jiangsu Province in China, (No. CXLX11_0735)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 3233-3240 )
英文信息