In vivo tracking of neuronal-like cells by magnetic resonance in rabbit models of spinal cord injury
Ruiping Zhang
Kun Zhang
Jianding Li
Qiang Liu
Jun Xie
摘要：In vitro experiments have demonstrated that neuronal-like cells derived from bone marrow mesenchymal stem cells can survive, migrate, integrate and help to restore the function and be-haviors of spinal cord injury models, and that they may serve as a suitable approach to treating spinal cord injury. However, it is very difficult to track transplanted cells in vivo. In this study, we in-jected superparamagnetic iron oxide-labeled neuronal-like cells into the subarachnoid space in a rabbit model of spinal cord injury. At 7 days after celltransplantation, a smal number of dot-shaped low signal intensity shadows were observed in the spinal cord injury region, and at 14 days, the number of these shadows increased on T2-weighted imaging. Perl’s Prussian blue staining de-tected dot-shaped low signal intensity shadows in the spinal cord injury region, indicative of superparamagnetic iron oxide nanoparticle-labeled cells. These findings suggest that transplanted neuronal-like cells derived from bone marrow mesenchymal stem cells can migrate to the spinal cord injury region and can be tracked by magnetic resonance in vivo. Magnetic resonance imaging represents an efficient noninvasive technique for visual y tracking transplanted cells in vivo.
机标关键词：spinal cord injurymarrow mesenchymal stem cellsmagnetic resonanceiron oxidePrussian blue
资助基金：This study was supported by a grant from Science and Technology Research Projects of Shanxi Province, (No.20120321028-02)a grant from the Scientific and Technical Foundation of Shanxi Provincial Health Department, (No.201201067)a grant from University Research and Development Projects of Shanxi Province, (No.20131101)grant from the National Natural Science Foundation of China, (No.81371628)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 3373-3381 )
英文信息