Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane
Shengzhong Ma
Changliang Peng
Shiqing Wu
Dongjin Wu
Chunzheng Gao
摘要：Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane pro-vides a good microenvironment for peripheral nerve regeneration;however, the precise mechanism remains unclear. p75 neurotrophin receptor (p75NTR) plays an important role in the regulation of pe-ripheral nerve regeneration. We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75NTR expression. In this study, we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve. Through RT-PCR and western blot analysis, nerve growth factor-containing fibrin glue membrane significantly increased p75NTR mRNA and protein expression in the Schwann cells at the anasto-motic site, in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture. These results indicate that nerve growth factor-containing fibrin glue membrane can promote pe-ripheral nerve regeneration by up-regulating p75NTR expression in Schwann cells.
机标关键词：fibrin gluenerve growth factorperipheral nerve regenerationsciatic nerveSchwann cellsnerve conduitwestern blot analysisprotein expression
资助基金：the Natural Science Foundation of Shandong Province in China, (No. ZR2013HM102, Y2007C046)the Promotive Research Fund for Excel ent Young and Middle-aged Scientists of Shandong Province in China, (No. BS2013YY038)the National Natural Science Foundation of China, (No.81301727)
论文发表日期：2013-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 3416-3422 )