Mash1 efifciently reprograms rat astrocytes into neurons
Daofang Ding
Leqin Xu
Hao Xu
Xiaofeng Li
Qianqian Liang
Yongjian Zhao
Yongjun Wang
摘要：To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mash1 and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mash1 was not detectable. Thus, we hypothesized that Mash1 alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mash1. One week later, we observed the changes in morphology of astrocytes, which showed typical neuro-nal characteristics. Moreover,β-tubulin expression levels were signiifcantly higher in astrocytes expressing Mash1 than in control cells. These results indicate that Mash1 alone can reprogram astrocytes into neurons.
机标关键词：
资助基金：This study was supported by the National Basic Research Program of China(973 Pro-gram)the Key Project of National Natural Science Foundation of China, (No.30930111)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 25-32 )
英文信息