Electro-acupuncture at Conception and Governor vessels and transplantation of umbilical cord blood-derived mesenchymal stem cells for treating cerebral ischemia/reperfusion injur y
Haibo Yu
Pengdian Chen
Zhuoxin Yang
Wenshu Luo
Min Pi
Yonggang Wu
Ling Wang
摘要：Mesenchymal stem cell transplantation is a novel means of treating cerebral ischemia/reper-fusion, and can promote angiogenesis and neurological functional recovery. Acupuncture at Conception and Governor vessels also has positive effects as a treatment for cerebral ischemia/reperfusion. Therefore, we hypothesized that electro-acupuncture at Conception and Governor vessels plus mesenchymal stem cell transplantation may have better therapeutic effects on the promotion of angiogenesis and recovery of neurological function than either treatment alone. In the present study, human umbilical cord blood-derived mesenchymal stem cells were isolated, cultured, identified and intracranially transplanted into the striatum and subcortex of rats at 24 hours following cerebral ischemia/reperfusion. Subsequently, rats were electro-acupunctured at Conception and Governor vessels at 24 hours after transplantation. Modified neurological severity scores and immunohistochemistry ifndings revealed that the combined interventions of electro-acupuncture and mesenchymal stem cell transplantation clearly improved neurological impairment and up-regulated vascular endothelial growth factor expression around the isch-emic focus. The combined intervention provided a better outcome than mesenchymal stem cell transplantation alone. These ifndings demonstrate that electro-acupuncture at Conception and Governor vessels and mesenchymal stem cell transplantation have synergetic effects on promot-ing neurological function recovery and angiogenesis in rats after cerebral ischemia/reperfusion.
机标关键词：cerebral ischemiastem cell transplantationgrowth factor expressionmesenchymal stem cellstherapeutic effectsfunction recovery
资助基金：This study was supported by the National Natural Science Foundation of China, (No.81072877)Key Laboratory Project of Condition and Platform Construction Plan of Shenzhen Sci-entific Research Fund, (No. CXB201111250113A)Shenzhen Scientific and Technology Development Program, (No.201203149)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 84-91 )