Regulatory effects of inhibiting the activation of glial cells on retinal synaptic plasticity
Lihong Zhou
Hui Wang
Jia Luo
Kun Xiong
Leping Zeng
Dan Chen
Jufang Huang
摘要：Various retinal injuries induced by ocular hypertension have been shown to induce plastic chang-es in retinal synapses, but the potential regulatory mechanism of synaptic plasticity after retinal injury was still unclear. A rat model of acute ocular hypertension was established by injecting saline intravitreally for an hour, and elevating the intraocular pressure to 14.63 kPa (110 mmHg). Western blot assay and immunolfuorescence results showed that synaptophysin expression had a distinct spatiotemporal change that increased in the inner plexiform layer within 1 day and spread across the outer plexiform layer after 3 days. Glial ifbrillary acidic protein expression in retinae was greatly increased after 3 days, and reached a peak at 7 days, which was also consistent with the peak time of synaptophysin expression in the outer plexiform layer following the in-creased intraocular pressure. Fluorocitrate, a glial metabolic inhibitor, was intravitreally injected to inhibit glial cell activation following high intraocular pressure. This signiifcantly inhibited the enhanced glial ifbrillary acidic protein expression induced by high intraocular pressure injury. Synaptophysin expression also decreased in the inner plexiform layer within a day and the wid-ened distribution in the outer plexiform layer had disappeared by 3 days. The results suggested that retinal glial cell activation might play an important role in the process of retinal synaptic plasticity induced by acute high intraocular pressure through affecting the expression and distri-bution of synaptic functional proteins, such as synaptophysin.
机标关键词：intraocular pressureocular hypertensionprotein expressionglial cellrat modelpeak time
资助基金：This study was supported by the National Natural Science Foundation of China, (No.81070729)the Natural Science Foundation of Hunan Province in China, (No.10JJ4023)the Hunan Provincial Innovation Foundation for Postgraduate in China, (No. CX2011B047)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 385-393 )
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