The Pael-R gene does not mediate the changes in rotenone-induced Parkinson’s disease model cells
Ting Zou
Xiangqi Tang
Zhiling Huang
Niangui Xu
Zhiping Hu
摘要：In this study, we established cell models for Parkinson’s disease using rotenone. An RNA interfer-ence vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ signiifcantly between groups, as de-tected by lfow cytometry and an MTT assay. These experimental ifndings indicate that the Pael-R gene has no role in the changes in rotenone-induced Parkinson’s disease model cells.
机标关键词：polymerase chain reactionwestern blot analysisRNA interferencedisease modelMTT assay
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 402-406 )
英文信息