7.0T nuclear magnetic resonance evaluation of the amyloid beta (1-40) animal model of Alzheimer’s disease:comparison of cytology veriifcation
Lei Zhang
Shuai Dong
Guixiang Zhao
Yu Ma
摘要：3.0T magnetic resonance spectroscopic imaging is a commonly used method in the research of brain function in Alzheimer’s disease. However, the role of 7.0T high-ifeld magnetic resonance spectroscopic imaging in brain function of Alzheimer’s disease remains unclear. In this study, 7.0T magnetic resonance spectroscopy showed that in the hippocampus of Alzheimer’s disease rats, the N-acetylaspartate wave crest was reduced, and the creatine and choline wave crest was elevated. This ifnding was further supported by hematoxylin-eosin staining, which showed a loss of hippocampal neurons and more glial cells. Moreover, electron microscopy showed neuronal shrinkage and mitochondrial rupture, and scanning electron microscopy revealed small size hippocampal synaptic vesicles, incomplete synaptic structure, and reduced number. Overall, the results revealed that 7.0T high-ifeld nuclear magnetic resonance spectroscopy detected the lesions and functional changes in hippocampal neurons of Alzheimer’s disease rats in vivo, allowing the possibility for assessing the success rate and grading of the amyloid beta (1-40) animal model of Alzheimer’s disease.
机标关键词：nuclear magnetic resonancehippocampal neuronsscanning electron microscopybrain functionwave crestsynaptic vesicles
资助基金：This study was supported by the National Natural Science Foundation of China, (No.81141013)a grant for Talents in Beijing, (No.2011D003034000019)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 430-435 )
英文信息