rAAV/ABAD-DP-6His attenuates oxidative stress-induced injur y of PC12 cells
Mingyue Jia
Mingyu Wang
Yi Yang
Yixin Chen
Dujuan Liu
Xu Wang
Lei Song
Jiang Wu
Yu Yang
摘要：Our previous studies have revealed that amyloidβ(Aβ)-binding alcohol dehydrogenase (ABAD) decoy peptide antagonizes Aβ42-induced neurotoxicity. However, whether it improves oxidative stress injury remains unclear. In this study, a recombinant adenovirus constitutively secreting and expressing Aβ-ABAD decoy peptide (rAAV/ABAD-DP-6His) was successfully constructed. Our results showed that rAAV/ABAD-DP-6His increased superoxide dismutase activity in hydro-gen peroxide-induced oxidative stress-mediated injury of PC12 cells. Moreover, rAAV/ABAD-DP-6His decreased malondialdehyde content, intracellular Ca2+concentration, and the level of reactive oxygen species. rAAV/ABAD-DP-6His maintained the stability of the mitochondrial membrane potential. In addition, the ATP level remained constant, and apoptosis was reduced. Overall, the results indicate that rAAV/ABAD-DP-6His generates the fusion peptide, Aβ-ABAD decoy peptide, which effectively protects PC12 cells from oxidative stress injury induced by hy-drogen peroxide, thus exerting neuroprotective effects.
机标关键词：oxidative stresssuperoxide dismutase activityreactive oxygen speciesneuroprotective effectsrecombinant adenovirusalcohol dehydrogenasemembrane potentialfusion peptide
资助基金：This study was supported by the National Natural Science Foundation of China for the Youth, (No.30800338,30801211)the National Natural Science Foundation of China, (No.30872721)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 481-488 )
英文信息