Transient axonal glycoprotein-1 induces apoptosis-related gene expression without triggering apoptosis in U251 glioma cells
Haigang Chang
Shanshan Song
Zhongcan Chen
Yaxiao Wang
Lujun Yang
Mouxuan Du
Yiquan Ke
Ruxiang Xu
Baozhe Jin
Xiaodan Jiang
摘要：Previous studies show that transient axonal glycoprotein-1, a ligand of amyloid precursor pro-tein, increases the secretion of amyloid precursor protein intracellular domain and is involved in apoptosis in Alzheimer’s disease. In this study, we examined the effects of transient axonal glyco-protein-1 on U251 glioma cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that transient axonal glycoprotein-1 did not inhibit the proliferation of U251 cells, but promoted cell viability. The terminal deoxynucleotidyl transferase dUTP nick end labeling assay showed that transient axonal glycoprotein-1 did not induce U251 cell apoptosis. Real-time PCR revealed that transient axonal glycoprotein-1 substantially upregulated levels of amyloid precursor protein intracellular C-terminal domain, and p53 and epidermal growth factor recep-tor mRNA expression. Thus, transient axonal glycoprotein-1 increased apoptosis-related gene expression in U251 cells without inducing apoptosis. Instead, transient axonal glycoprotein-1 promoted the proliferation of these glioma cells.
机标关键词：glioma cellsamyloid precursor proteinepidermal growth factormRNA expressiongene expressioncell viabilitycell apoptosis
资助基金：grants from the National Natural Science Foundation of China, (No.81171179,81272439)the Key Sci-Tech Research Projects of Guangdong Province in China, (No.2008A030201019)the Guangzhou Municipal Science and Technology Project in China, (No.09B52120112-2009J1-C418-2, No.2008A1-E4011-6)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 519-525 )
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