Preconditioning crush increases the survival rate of motor neurons after spinal root avulsion
Lin Li
Yizhi Zuo
Jianwen He
摘要：In a previous study, heat shock protein 27 was persistently upregulated in ventral motor neurons following nerve root avulsion or crush. Here, we examined whether the upregulation of heat shock protein 27 would increase the survival rate of motor neurons. Rats were divided into two groups:an avulsion-only group (avulsion of the L4 lumbar nerve root only) and a crush-avulsion group (the L4 lumbar nerve root was crushed 1 week prior to the avulsion). Immunofluores-cent staining revealed that the survival rate of motor neurons was significantly greater in the crush-avulsion group than in the avulsion-only group, and this difference remained for at least 5 weeks after avulsion. The higher neuronal survival rate may be explained by the upregulation of heat shock protein 27 expression in motor neurons in the crush-avulsion group. Further-more, preconditioning crush greatly attenuated the expression of nitric oxide synthase in the motor neurons. Our ifndings indicate that the neuroprotective action of preconditioning crush is mediated through the upregulation of heat shock protein 27 expression and the attenuation of neuronal nitric oxide synthase upregulation following avulsion.
机标关键词：motor neuronsheat shock proteinneuronal nitric oxide synthasesurvival ratenerve root
资助基金：This study was supported by a grant from Education Ministry of Jiangsu Province, (No.08KJB310002)Excellent Discipline of Jiangsu Province, (No. JX10131801096)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 540-548 )
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