Neuronal apoptosis in cerebral ischemia/reperfusion area following electrical stimulation of fastigial nucleus
Jingli Liu
Jinpin Li
Yi Yang
Xiaoling Wang
Zhaoxia Zhang
Lei Zhang
摘要：Previous studies have indicated that electrical stimulation of the cerebellar fastigial nucleus in rats may reduce brain infarct size, increase the expression of Ku70 in cerebral ischemia/reperfusion area, and decrease the number of apoptotic neurons. However, the anti-apoptotic mechanism of Ku70 remains unclear. In this study, fastigial nucleus stimulation was given to rats 24, 48, and 72 hours before cerebral ischemia/reperfusion injury. Results from the electrical stim-ulation group revealed that rats exhibited a reduction in brain infarct size, a signiifcant increase in the expression of Ku70 in cerebral ischemia/reperfusion regions, and a decreased number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells. Double immunofluorescence staining revealed no co-localization of Ku70 with TUNEL-positive cells. However, Ku70 partly co-localized with Bax protein in the cytoplasm of rats with cerebral ischemia/reperfusion injury. These ifndings suggest an involvement of Ku70 with Bax in the cy-toplasm of rats exposed to electrical stimulation of the cerebellar fastigial nucleus, and may thus provide an understanding into the anti-apoptotic activity of Ku70 in cerebral ischemia/reperfu-sion injury.
机标关键词：cerebral ischemiacerebellar fastigial nucleuselectrical stimulationBax protein
资助基金：This study was financially supported by the National Natural Science Foundation of China, (No.30860291)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 727-734 )
英文信息