Houshiheisan compound prescription protects neurovascular units after cerebral ischemia
Haizheng Wang
Lei Wang
Nan Zhang
Qi Zhang
Hui Zhao
Qiuxia Zhang
摘要：Houshiheisan is composed of wind-dispelling (chrysanthemun lfower, divaricate saposhnikovia root, Manchurian wild ginger, cassia twig, Szechwan lovage rhizome, and platycodon root) and deifciency-nourishing (ginseng, Chinese angelica, large-head atractylodes rhizome, Indian bread, and zingiber) drugs. In this study, we assumed these drugs have protective effects against cerebral ischemia, on neurovascular units. Houshiheisan was intragastrically administered in a rat model of focal cerebral ischemia. Hematoxylin-eosin staining, transmission electron microscopy, immu-nolfuorescence staining, and western blot assays showed that Houshiheisan reduced pathological injury to the ischemic penumbra, protected neurovascular units, visibly up-regulated neuronal nuclear antigen expression, and down-regulated amyloid precursor protein and amyloid-β42 expression. Wind-dispelling and deifciency-nourishing drugs maintained NeuN expression to varying degrees, but did not affect amyloid precursor protein or amyloid-β42 expression in the ischemic penumbra. Our results suggest that the compound prescription Houshiheisan effectively suppresses abnormal amyloid precursor protein accumulation, reduces amyloid substance depo-sition, maintains stabilization of the internal environment of neurovascular units, and minimizes injury to neurovascular units in the ischemic penumbra.
机标关键词：amyloid precursor proteinischemic penumbrafocal cerebral ischemiaelectron microscopyprotective effectsantigen expressionwestern blotrat model
资助基金：This study was supported by the National Natural Science Foundation of China, (No.30973782,81373526)the Natural Science Foundation of Beijing, (No.7102014,7122018)the Beijing Municipal Higher Learning Institution Talent Teaching Plan“Young and Middle-aged Talented People Training”Project, (No. PXM2011014226)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 741-748 )
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