Fusion protein of single-chain variable domain fragments for treatment of myasthenia gravis
Fangfang Li
Fanping Meng
Quanxin Jin
Changyuan Sun
Yingxin Li
Honghua Li
Songzhu Jin
摘要：Single-chain variable domain fragment (scFv) 637 is an antigen-specific scFv of myasthenia gravis. In this study, scFv and human serum albumin genes were conjugated and the fusion pro-tein was expressed in Pichia pastoris. The afifnity of scFv-human serum albumin fusion protein to bind to acetylcholine receptor at the neuromuscular junction of human intercostal muscles was detected by immunolfuorescence staining. The ability of the fusion protein to block myas-thenia gravis patient sera binding to acetylcholine receptors and its stability in healthy serum were measured by competitive ELISA. The results showed that the inhibition rate was 2.0-77.4%, and the stability of fusion protein in static healthy sera was about 3 days. This approach suggests the scFv-human serum albumin is a potential candidate for speciifc immunosuppressive therapy of myasthenia gravis.
机标关键词：human serum albuminfusion proteinmyasthenia gravisimmunosuppressive therapyacetylcholine receptorPichia pastorisinhibition rate
资助基金：the National Natural Science Foundation of China, (No.30360100,30760234,30860260,81160373,81360458)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 851-856 )
英文信息