Regulator y effects of anandamide on intracellular Ca2+concentration increase in trigeminal ganglion neurons
Yi Zhang
Hong Xie
Gang Lei
Fen Li
Jianping Pan
Changjin Liu
Zhiguo Liu
Lieju Liu
Xuehong Cao
摘要：Activation of cannabinoid receptor type 1 on presynaptic neurons is postulated to suppress neu-rotransmission by decreasing Ca2+influx through high voltage-gated Ca2+channels. However, recent studies suggest that cannabinoids which activate cannabinoid receptor type 1 can increase neurotransmitter release by enhancing Ca2+influx in vitro. The aim of the present study was to investigate the modulation of intracellular Ca2+concentration by the cannabinoid receptor type 1 agonist anandamide, and its underlying mechanisms. Using whole cell voltage-clamp and calcium imaging in cultured trigeminal ganglion neurons, we found that anandamide directly caused Ca2+inlfux in a dose-dependent manner, which then triggered an increase of intracellular Ca2+concentration. The cyclic adenosine and guanosine monophosphate-dependent protein kinase systems, but not the protein kinase C system, were involved in the increased intracellular Ca2+concentration by anandamide. This result showed that anandamide increased intracellu-lar Ca2+concentration and inhibited high voltage-gated Ca2+channels through different signal transduction pathways.
机标关键词：protein kinase Ctrigeminal ganglionsignal transductioncalcium imagingin vitro
资助基金：NIH, grant (No. GM-63577)NNSF, grant (No.30571537, No.30271500)the National Natural Science Foundation of China, (No.30271500,30571537 and 81370246.2010 National Clinical Key Disciplines Construction Grant from the Ministry of Health of the People’s Republic of China)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 878-887 )
英文信息