Dynamic culture of a thermosensitive collagen hydrogel as an extracellular matrix improves the construction of tissue-engineered peripheral nerve
Lanfeng Huang
Rui Li
Wanguo Liu
Jin Dai
Zhenwu Du
Xiaonan Wang
Jianchao Ma
Jinsong Zhao
摘要：Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, but cell loss between seeding and adhesion to the scaffold remains inevitable. A thermo-sensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve compos-ites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesen-chymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to ifll in the pores, and seeded cells adhered onto the poly-L-lactic acid ifbers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive colla-gen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermo-sensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material.
机标关键词：peripheral nerve injurymarrow mesenchymal stem cellsbone marrowDNA contentextracellular matrixcell lossin vitro
资助基金：This study was supported by the National Natural Science Foundation of China, (No.31071222)Jilin Province Science and Technology Development Project in China, (No.20080738)the Frontier Interdiscipline Program of Norman Bethune Health Science Center of Jilin University in China, (No.2013106023)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1371-1378 )
英文信息