Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injur y by promoting axonal growth and anti-autophagy
Fei Yin
Chunyang Meng
Rifeng Lu
Lei Li
Ying Zhang
Hao Chen
Yonggang Qin
Li Guo
摘要：Bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after trans-plantation in the spinal cord of rats with ischemia/reperfusion injury. Although bone marrow mesenchymal stem cells are known to protect against spinal cord ischemia/reperfusion injury through anti-apoptotic effects, the precise mechanisms remain unclear. In the present study, bone marrow mesenchymal stem cells were cultured and proliferated, then transplanted into rats with ischemia/reperfusion injury via retro-orbital injection. Immunohistochemistry and immunolfuorescence with subsequent quantiifcation revealed that the expression of the axonal regeneration marker, growth associated protein-43, and the neuronal marker, microtubule-as-sociated protein 2, significantly increased in rats with bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Fur-thermore, the expression of the autophagy marker, microtubule-associated protein light chain 3B, and Beclin 1, was signiifcantly reduced in rats with the bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Western blot analysis showed that the expression of growth associated protein-43 and neuro-iflament-H increased but light chain 3B and Beclin 1 decreased in rats with the bone marrow mesenchymal stem cell transplantation. Our results therefore suggest that bone marrow mes-enchymal stem cell transplantation promotes neurite growth and regeneration and prevents autophagy. These responses may likely be mechanisms underlying the protective effect of bone marrow mesenchymal stem cells against spinal cord ischemia/reperfusion injury.
机标关键词：spinal cord ischemiamarrow mesenchymal stem cellsstem cell transplantationbone marrowaxonal regenerationprotective effect
资助基金：This study was supported by the National Natural Science Foundation of China, (No.30972153)the Science and Technology Development Program of Jilin Provincial Science and Tech-nology Department in China, (No.200905183)the Scientific Research Foundation of Jilin Department of Health of China, (No.2008Z041)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 1665-1671 )
英文信息