Oxidative phosphor ylated neuroiflament protein M protects spinal cord against ischemia/reperfusion injury
Haitao Wang
Su Pan
Xiaoyu Yang
Benqing Zhu
Dalin Wang
摘要：Previous studies have shown that neuroiflament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec-trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen-sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most signiifcant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neuroiflament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neuroiflament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neuroiflament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.
机标关键词：western blot analysisneurofilament proteinspinal cord ischemiagel electrophoresisprotein expressionoxidative phosphorylationmass spectrometryoxidative stress
资助基金：This study was financially supported by the National Natural Science Foundation of China, (No.81350013,30872609)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 1672-1677 )
英文信息