Amyloid precursor-like protein 2 C-terminal fragments upregulateS100A9 gene and protein expression in BV2 cells
Guangzhe Li
Hui Chen
Lin Cheng
Rongjie Zhao
Junchang Zhao
Yanji Xu
摘要：The murine microglial cell line BV2 has neuroprotective effects, but is toxic to neurons by secret-ing inlfammatory cytokines, and is an important target in the treatment of nerve inlfammation and neurodegenerative diseases. In the present study, we observed the effects of transfecting three amyloid precursor-like protein 2 (APLP2) C-terminal fragments (CTFs; C57, C50 and C31) in the pEGFP-N1 vector on S100A9 expression in BV2 cells. Reverse transcription-PCR, western blot assay and immunocytochemistry revealed that S100A9 protein and mRNA expression was greater in BV2 cells after CTF transfection than after mock transfection with an empty vector. Furthermore, transfection of full-length APLP2-751 resulted in low levels of S100A9 protein ex-pression. Our results show that APLP2-CTFs upregulate S100A9 protein and mRNA expression in BV2 cells, and identify a novel pathway involved in neuronal injury and apoptosis, and repair and protection in Alzheimer’s disease.
机标关键词：mRNA expressionneurodegenerative diseasesneuroprotective effectsneuronal injurycell line
资助基金：Funding(No.2011-15237)the National Natural Science Foundation of China, (No.81160159)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 1923-1928 )
英文信息