Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Qing Li
Jiang Lu
Xianyu Wang
摘要：Propofol and remifentanil alter intracellular Ca2+ concentration ([Ca2+]i) in neural stem/progen-itor cells by activating γ-aminobutyric acid type A receptors and by reducing testosterone levels. However, whether this process affects neural stem/progenitor cell proliferation and differenti-ation remains unknown. In the present study, we applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations (1, 2–2.5 and 4–5 times the clinically effective blood drug concentration), to neural stem/progenitor cells from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed neural stem/progenitor cell proliferation and differen-tiation, and induced a decrease in [Ca2+]i during the initial stage of neural stem/progenitor cell differentiation. We therefore propose that propofol and remifentanil interfere with the prolifer-ation and differentiation of neural stem/progenitor cells by altering [Ca2+]i. Our ifndings suggest that propofol and/or remifentanil should be used with caution in pediatric anesthesia.
机标关键词：cell proliferationcell differentiationinitial stagerat pupstype A
资助基金：Funding(No.2012FFC060)the Natural Science Foundation of Hubei University of Medicine in China, (No.2011QDZR-2)the Provincial Key Disciplines Foundation of Hubei Province of China, (No.2014XKJSSJ04)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 2002-2007 )
英文信息