The Achyranthes bidentata polypeptide k fraction enhances neuronal growth in vitro and promotes peripheral nerve regeneration after crush injury in vivo
Qiong Cheng
Chunyi Jiang
Caiping Wang
Shu Yu
Qi Zhang
Xiaosong Gu
Fei Ding
摘要：We have previously shown that Achyranthes bidentata polypeptides (ABPP), isolated from Achy-ranthes bidentata Blume (a medicinal herb), exhibit neurotrophic and neuroprotective effects on the nervous system. To identify the major active component of ABPP, and thus optimize the use of ABPP, we used reverse-phase high performance liquid chromatography to separate ABPP. We ob-tained 12 fractions, among which the fraction of ABPPk demonstrated the strongest neuroactivity. Immunocytochemistry and western blot analysis showed that ABPPk promoted neurite growth in cultured dorsal root ganglion explant and dorsal root ganglion neurons, which might be associated with activation of Erk1/2. A combination of behavioral tests, electrophysiological assessment, and histomorphometric analysis indicated that ABPPk enhanced nerve regeneration and function res-toration in a mouse model of crushed sciatic nerve. All the results suggest that ABPPk, as the key component of ABPP, can be used for peripheral nerve repair to yield better outcomes than ABPP.
机标关键词：crush injuryperipheral nerve regenerationdorsal root ganglion neuronshigh performance liquid chromatographyneuroprotective effectswestern blot analysisnervous systemmedicinal herb
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 2142-2150 )
英文信息