Tracking of iron-labeled human neural stem cells by magnetic resonance imaging in cell replacement therapy for Parkinson’s disease
Milagros Ramos-Gmez
Alberto Martnez-Serrano
摘要：Human neural stem cells (hNSCs) derived from the ventral mesencephalon are powerful research tools and candidates for cell therapies in Parkinson’s disease. However, their clinical translation has not been fully realized due, in part, to the limited ability to track stem cell regional localization and survival over long periods of time afterin vivo transplantation. Magnetic resonance imaging provides an excellent non-invasive method to study the fate of transplanted cellsin vivo. For magnetic resonance imaging cell tracking, cells need to be labeled with a contrast agent, such as magnetic nanoparticles, at a concentration high enough to be easily detected by magnetic resonance imaging. Grafting of human neural stem cells labeled with magnetic nanoparticles allows cell tracking by magnetic resonance imaging without impairment of cell survival, prolif-eration, self-renewal, and multipotency. However, the results reviewed here suggest that in long term grafting, activated microglia and macrophages could contribute to magnetic resonance imaging signal by engulifng dead labeled cells or iron nanoparticles dispersed freely in the brain parenchyma over time.
机标关键词：replacement therapymagnetic resonance imagingmagnetic nanoparticlesneural stem cellsiron nanoparticlescontrast agentcell survival
资助基金：Work at the author’s laboratories was supported by(RETICS TerCel RD12/0019/0013)Co-munidad Autónoma de Madrid(S2010-BMD-2336)MINECO(SAF2010-17167)the institutional grant of the Fundación Ramón Areces to the CBMSO. To MRG(S2010-BMD-2460)
论文发表日期：2016-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 49-52 )
英文信息