Synthetic cell pathobiology to study neurodegeneration: defining new therapeutic targets in astroglia
Matja(z) Stenovec
Robert Zorec
摘要：Synthetic biology is the design and construction of new biological parts,devices,and systems,and the re-design of existing,natural biological systems for useful purposes.This new interdisciplinary effort has gained interest from the industry sector,and synthetic biology has been used to produce fragrances,such as vanillin in yeast,in cells that do not normally exist in nature (Carlquist et al.,2015).Moreover,to simplify the process of developing new drugs,which requires many years and significant resources,testing in animal disease models could be replaced by engineered cells that represent and underlie the function of an organ.High content omic techniques in combination with stable human in vitro cell culture systems can be used to improve pre-clinical safety regimes by providing detailed mechanistic information on altered cellular processes.In cultured human renal epithelial cells,the mechanism of action of the nephrotoxin cyclosporine A was quantified by liquid chromatography-tandem mass spectrometry for kinetic modeling,and this approach appeared useful in determining the key biological properties of epithelial cells such as cell metabolism and the proteins required for the formation of proximal tubule epithelia (Aschauer et al.,2013;Wilmes et al.,2013).
机标关键词：epithelial cellsdesign and constructionbiological propertiesanimal disease modelsmechanism of actionbiological systemsmass spectrometrykinetic modeling
论文发表日期：2016-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：2( 234-235 )
英文信息