Time-lapse changes of in vivo injured neuronal substructures in the central nervous system after low energy two-photon nanosurgery
Zhikai Zhao
Shuangxi Chen
Yunhao Luo
Jing Li
Smaranda Badea
Chaoran Ren
摘要：There is currently very little research regarding the dynamics of the subcellular degenerative events that occur in the central nervous system in response to injury. To date, multi-photon excitation has been primarily used for imaging applications;however, it has been recently used to selectively disrupt neural structures in living animals. However, understanding the complicated processes and the essential underly-ing molecular pathways involved in these dynamic events is necessary for studying the underlying process that promotes neuronal regeneration. In this study, we introduced a novel method allowing in vivo use of low energy (less than 30 mW) two-photon nanosurgery to selectively disrupt individual dendrites, axons, and dendritic spines in the murine brain and spinal cord to accurately monitor the time-lapse changes in the injured neuronal structures. Individual axons, dendrites, and dendritic spines in the brain and spinal cord were successfully ablated and in vivo imaging revealed the time-lapse alterations in these structures in response to the two-photon nanosurgery induced lesion.The energy (less than 30 mW) used in this study was very low and caused no observable additional damage in the neuronal sub-structures that occur fre-quently, especially in dendritic spines, with current commonly used methods using high energy levels. In addition, our approach includes the option of monitoring the time-varying dynamics to control the degree of lesion.The method presented here may be used to provide new insight into the growth of axons and den-drites in response to acute injury.
机标关键词：spinal cordcentral nervous systemmurine brainthe growth
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 751-756 )
英文信息