Metabolite changes in the ipsilateral and contralateral cerebral hemispheres in rats with middle cerebral artery occlusion
Lei Ruan
Yan Wang
Shu-chao Chen
Tian Zhao
Qun Huang
Zi-long Hu
Neng-zhi Xia
Jin-jin Liu
Wei-jian Chen
Yong Zhang
Jing-liang Cheng
Hong-chang Gao
Yun-jun Yang
Hou-zhang Sun
摘要：Cerebral ischemia not only causes pathological changes in the ischemic areas but also induces a series of secondary changes in more distal brain regions (such as the contralateral cerebral hemisphere). The impact of supratentorial lesions, which are the most common type of lesion, on the contralateral cerebellum has been studied in patients by positron emission tomography, single photon emission computed tomography, magnetic resonance imaging and diffusion tensor imaging. In the present study, we investigated metabolite changes in the contralateral cerebral hemisphere after supratentorial unilateral ischemia using nuclear magnetic resonance spectroscopy-based metabo-nomics. The permanent middle cerebral artery occlusion model of ischemic stroke was established in rats. Rats were randomly divided into the middle cerebral artery occlusion 1-, 3-, 9- and 24-hour groups and the sham group. 1H nuclear magnetic resonance spectroscopy was used to detect metabolites in the left and right cerebral hemispheres. Compared with the sham group, the concentrations of lactate, alanine,γ-aminobutyric acid, choline and glycine in the ischemic cerebral hemisphere were increased in the acute stage, while the concentrations of N-acetyl aspartate, creatinine, glutamate and aspartate were decreased. This demonstrates that there is an upregulation of anaerobic glycol-ysis (shown by the increase in lactate), a perturbation of choline metabolism (suggested by the increase in choline), neuronal cell damage (shown by the decrease in N-acetyl aspartate) and neurotransmitter imbalance (evidenced by the increase in γ-aminobutyric acid and glycine and by the decrease in glutamate and aspartate) in the acute stage of cerebral ischemia. In the contralateral hemisphere, the concen-trations of lactate, alanine, glycine, choline and aspartate were increased, while the concentrations of γ-aminobutyric acid, glutamate and creatinine were decreased. This suggests that there is a difference in the metabolite changes induced by ischemic injury in the contralateral and ipsilateral cerebral hemispheres. Our findings demonstrate the presence of characteristic changes in metabolites in the contralateral hemisphere and suggest that they are most likely caused by metabolic changes in the ischemic hemisphere.
机标关键词：nuclear magnetic resonancemiddle cerebral arterycerebral ischemiapositron emission tomographydiffusion tensor imagingcomputed tomographysecondary changesischemic stroke
资助基金：grants from the Health Innovation Talents Project of Zhejiang Province of China, (No. 2016)the National Natural Science Foundation of China, (No. 81571626, U1404823)the Natural Science Foundation of Zhejiang Province of China, (No. LY15H220001)the Medical and Health Research Project of Zhejiang Province of China, (No. 2014KYA134)the Wenzhou Bureau of Science and Technology of China, (No. Y20140731, Y20150087)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 931-937 )
英文信息