Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Jiang Lu
Xue-qin Yao
Yu Wang
Sookja Kim Chung
He-xin Tang
Xian-yu Wang
Chen Meng
Qing Li
摘要：Monosialoganglioside 1 (GM1) is the main ganglioside subtype and has neuroprotective properties in the central nervous system. In this study, we aimed to determine whether GM1 alleviates neurotoxicity induced by moderate and high concentrations of propofol combined with remifentanil in the immature central nervous system. Hippocampal neural stem cells were isolated from newborn Sprague-Dawley rats and treated with remifentanil (5, 10, 20 ng/mL) and propofol (1.0, 2.5, 5.0 μg/mL), and/or GM1 (12.5, 25, 50 μg/mL). GM1 reversed combined propofol and remifentanil-induced decreases in the percentage of 5-bromodeoxyuridine(+) cells and also reversed the increase in apoptotic cell percentage during neural stem cell proliferation and differentiation. However, GM1 with combined propofol and remifen-tanil did not affect β-tubulin(+) or glial fibrillary acidic protein(+) cell percentage during neural stem cell differentiation. In conclusion, we show that GM1 alleviates the damaging effects of propofol combined with remifentanil at moderate and high exposure concentrations in neural stem cells in vitro, and exerts protective effects on the immature central nervous system.
机标关键词：central nervous systemneural stem cellscell differentiationprotective effectscell proliferationacidic proteinin vitro
资助基金：This study was supported by the Natural Science Foundation of Hubei Province of China, (No. 2012FFC060)the Natural Science Foundation of Hubei University of Medicine of China, (No. 2011QDZR-2)a grant from the Scientific and Technological Project of Shiyan City of Hubei Province of China, (No. 14Y13)a grant from the National College Student Innovation and Entrepreneurship Training Program of Ed-ucation Ministry of China, (No. 201610929005 and No. 201210929004)the Educational Scientific Foundation of Hubei University of Medicine of China in 2016, (No. 2016027)the Fund of Disciplines Construction of Hubei University of Medicine of China in 2015, (No. 2015)the Scientific Foundation of Affiliated Taihe Hospital of Hubei University of Medicine of China in 2016, (No. 2016JJXM001)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 945-952 )
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