Effect of hyperthermia on calbindin-D 28k immunoreactivity in the hippocampal formation following transient global cerebral ischemia in gerbils
Jae-Chul Lee
Jeong-Hwi Cho
Tae-Kyeong Lee
In Hye Kim
Moo-Ho Won
Geum-Sil Cho
Bich-Na Shin
In Koo Hwang
Joon Ha Park
Ji Hyeon Ahn
Il Jun Kang
Young Joo Lee
Yang Hee Kim
摘要：Calbindin D-28K (CB), a Ca2+-binding protein, maintains Ca2+ homeostasis and protects neurons against various insults. Hyperthermia can exacerbate brain damage produced by ischemic insults. However, little is reported about the role of CB in the brain under hyperthermic condition during ischemic insults. We inves-tigated the effects of transient global cerebral ischemia on CB immunoreactivity as well as neuronal damage in the hippocampal formation under hyperthermic condition using immunohistochemistry for neuronal nuclei (NeuN) and CB, and Fluoro-Jade B histofluorescence staining in gerbils. Hyperthermia (39.5 ± 0.2°C) was induced for 30 minutes before and during transient ischemia. Hyperthermic ischemia resulted in neu-ronal damage/death in the pyramidal layer of CA1–3 area and in the polymorphic layer of the dentate gyrus at 1, 2, 5 days after ischemia. In addition, hyperthermic ischemia significantly decreaced CB immunoreac-tivity in damaged or dying neurons at 1, 2, 5 days after ischemia. In brief, hyperthermic condition produced more extensive and severer neuronal damage/death, and reduced CB immunoreactivity in the hippocampus following transient global cerebral ischemia. Present findings indicate that the degree of reduced CB immu-noreactivity might be related with various neuronal damage/death overtime and corresponding areas after ischemic insults.
机标关键词：cerebral ischemiahippocampal formationdentate gyrusbrain damage
资助基金：This study was supported by the Biomedical Technology Development Program of the NRF funded by the Korean Government, MSIP(NRF-2015M3A9B6066835)by the Bio-Synergy Research Project (NRF-2015M3A9C4076322)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 1458-1464 )
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