Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia
Hyun-Jin Tae
Il Jun Kang
Tae-Kyeong Lee
Jeong Hwi Cho
Jae-Chul Lee
Myoung Cheol Shin
Yoon Sung Kim
Jun Hwi Cho
Jong-Dai Kim
Ji Hyeon Ahn
Joon Ha Park
In-Shik Kim
Hyang-Ah Lee
Yang Hee Kim
Moo-Ho Won
Young Joo Lee
摘要：Low survival rate occurs in patients who initially experience a spontaneous return of circulation after cardiac arrest (CA). In this study, we induced asphyxial CA in adult male Sprague-Daley rats, maintained their body temperature at 37 ± 0.5°C, and then observed the survival rate during the post-resuscitation phase. We examined neuronal damage in the hippocampus using cresyl violet (CV) and Fluore-Jade B (F-J B) staining, and pro-inflammatory response using ionized calcium-binding adapter molecule 1 (Iba-1), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-α) immunohistochemistry in the hippocampus after asphyxial CA in rats under normothermia. Our results show that the survival rate decreased gradually post-CA (about 63% at 6 hours, 37% at 1 day, and 8% at 2 days post-CA). Rats were sacrificed at these points in time post-CA, and no neuronal damage was found in the hippocampus until 1 day post-CA. However, some neurons in the stratum pyramidale of the CA region in the hippocampus were dead 2 days post-CA. Iba-1 immunoreactive microglia in the CA1 region did not change until 1 day post-CA, and they were activated (enlarged cell bodies with short and thicken processes) in all layers 2 days post-CA. Meanwhile, GFAP-immunoreactive astrocytes did not change significantly until 2 days post-CA. TNF-α immunoreactivity decreased significantly in neurons of the stratum pyramidale in the CA1 region 6 hours post-CA, decreased gradually until 1 day post-CA, and increased significantly again 2 days post-CA. These findings suggest that low survival rate of normothermic rats in the early period of asphyxia-induced CA is related to increased TNF-α immunoreactivity, but not to neuronal damage in the hippocampal CA1 region.
机标关键词：survival ratebody temperaturecardiac arrestacidic protein
资助基金：the Ministry of Education(NRF-2014R1A1A2057263)by the Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science)by the Basic Science Research Program through the National Research Foundation of Korea( ICT & Future Planning(NRF-2017R1A2B4009079 & NRF-2017R1A2B4008403)by the Bio-Synergy Research Project (NRF-2015M3A9C4076322)
论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 2007-2013 )
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