Does combined therapy of curcumin and epigallocatechin gallate have a synergistic neuroprotective effect against spinal cord injury?
Jiri Ruzicka
Lucia Machova Urdzikova
Barbora Svobodova
Anubhav G.Amin
Kristyna Karova
Jana Dubisova
Kristyna Zaviskova
Sarka Kubinova
Meic Schmidt
Meena Jhanwar-Uniyal
Pavia Jendelova
摘要：Systematic inflammatory response after spinal cord injury (SCI) is one of the factors leading to lesion development and a profound degree of functional loss.Anti-inflammatory compounds,such as curcumin and epigatlocatechin gallate (EGCG) are known for their neuroprotective effects.In this study,we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression.Immediately after SCI,rats received curcumin,EGCG,curcumin + EGCG or saline [daily intraperitoneal doses (curcumin,6 mg/kg;EGCG 17 mg/kg)] and weekly intramuscular doses (curcumin,60 mg/kg;EGCG 17 mg/kg)] for 28 days.Rats were evaluated using behavioral tests (the Basso,Beattie,and Bresnahan (BBB) open-field locomotor test,flat beam test).Spinal cord tissue was analyzed using histological methods (Luxol Blue-cresyl violet staining) and immunohistochemistry (anti-glial fibrillary acidic protein,anti-growth associated protein 43).Cytokine levels (interleukin-1β,interleukin-4,interleukin-2,interleukin-6,macrophage inflammatory protein 1-alpha,and RANTES) were measured using Luminex assay.Quantitative polymerase chain reaction was performed to determine the relative expression of genes (Sort1,Fgf2,Irf5,Mrc1,Olig2,Casp3,Gap43,Gfap,Vegf,NfκB,Cntf) related to regenerative processes in injured spinal cord.We found that all treatments displayed significant behavioral recovery,with no obvious synergistic effect after combined therapy of curcumin and ECGC.Curcumin and EGCG alone or in combination increased axonal sprouting,decreased glial scar formation,and altered the levels of macrophage inflammatory protein 1-alpha,interleukin-1β,interleukin-4 and interleukin-6 cytokines.These results imply that although the expected synergistic response of this combined therapy was less obvious,aspects of tissue regeneration and immune responses in severe SCI were evident.
机标关键词：
资助基金：registration number CZ.02.1.01/0.0./0.0/15_003/0000419 and project InterAction LTAUSA17120.All experiments were performed in accordance with the European Communities Council Directive of 22nd of September 2010 (2010/63/EU)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 119-127 )
英文信息