Catgut implantation at acupoints increases the expression of glutamate aspartate transporter and glial glutamate transporter-1 in the brain of rats with spasticity after stroke
Rui-Qing Li
Ming-Yue Wan
Jing Shi
Hui-Ling Wang
Fei-Lai Liu
Cheng-Mei Liu
Jin Huang
Ren-Chao Liu
Le Ma
Xiao-Dong Feng
摘要：Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui (GV14),Jizhong (GV6),Houhui,Guanyuan (CV4) and Zhongwan (CV12).The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter (GLAST) and glial glutamate transporter-1 (GLT-1) expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1 mRNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 mRNAs was increased on the injured (left) side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.
机标关键词：
资助基金：This study was supported by the National Natural Science Foundation of China,(No.14202225,&.81574042)the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China,(No.2000202)a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China,(No.JDZX2015314)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 1013-1018 )
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