Microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in dorsal root ganglia and neuropathic pain
Ya-Ling Zhang
De-Jian Chen
Bao-Lin Yang
Tao-Tao Liu
Jia-Juan Li
Xiu-Qi Wang
Guo-Yong Xue
Zeng-Xu Liu
摘要：Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelin-ation. Microcapsule technology largely mitigates immune rejection of transplanted cells. We previously showed that microencapsulated olfactory ensheathing cells can reduce neuropathic pain and we hypothesized that microencapsulated Schwann cells can also inhibit neu-ropathic pain. Rat Schwann cells were cultured by subculture and then microencapsulated and were tested using a rat chronic constriction injury (CCI) neuropathic pain model. CCI rats were treated with Schwann cells or microencapsulated Schwann cells and were compared with sham and CCI groups. Mechanical withdrawal threshold and thermal withdrawal latency were assessed preoperatively and at 1, 3, 5, 7, 9, 11 and 14 days postoperatively. The expression of P2X3 receptors in L4–5 dorsal root ganglia of the different groups was detected by double-label immunofluorescence on day 14 after surgery. Compared with the chronic constriction injury group, mechanical withdrawal threshold and thermal withdrawal latency were higher, but the expression of P2X3 receptors was remarkably decreased in rats treated with Schwann cells and microencapsulated Schwann cells, especially in the rats transplanted with microencapsulated Schwann cells. The above data show that microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in L4–5 dorsal root ganglia and neuro-pathic pain.
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资助基金：This study was supported by the National Natural Science Foundation of China, (No. 81760418 and 81260190)the Natural Science Foundation of Jiangxi Province, (No. 20132BAB205023, 20151BAB205022)a grant from Science and Technology Research Project of Jiangxi Education Department, (No. GJJ13159)a grant from the Science and Technology Program of Department of Health of Jiangxi Province, (No. 20173010)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 1961-1967 )
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