Effect of sevoflurane preconditioning on astrocytic dynamics and neural network formation after cerebral ischemia and reperfusion in rats
Qiong Yu
Li Li
Wei-Min Liang
摘要：Astrocytes,the major component of blood-brain barriers,have presented paradoxical profiles after cerebral ischemia and reperfusion in vivo and in vitro.Our previous study showed that sevoflurane preconditioning improved the integrity of blood-brain barriers after ischemia and reperfusion injury in rats.This led us to investigate the effects of sevoflurane preconditioning on the astrocytic dynamics in ischemia and reperfusion rats,in order to explore astrocytic cell-based mechanisms of sevoflurane preconditioning.In the present study,2,3,5-triphenyltetrazolium chloride staining and Garcia behavioral scores were utilized to evaluate cerebral infarction and neurological outcome from day 1 to day 3 after transient middle cerebral artery occlusion surgery.Using immunofluorescent staining,we found that sevoflurane precondi-tioning substantially promoted the astrocytic activation and migration from the penumbra to the infarct with microglial activation from day 3 after middle cerebral artery occlusion.The formation of astrocytic scaffolds facilitated neuroblasts migrating from the subventricular zone to the lesion sites on day 14 after injury.Neural networks increased in the infarct of sevoflurane preconditioned rats,consistent with decreased infarct volume and improved neurological scores after ischemia and reperfusion injury.These findings demonstrate that sevoflurane preconditioning confers neuroprotection,not only by accelerating astrocytic spatial and temporal dynamics,but also providing astrocytic scaffolds for neuroblasts migration to ischemic regions,which facilitates neural reconstruction after brain ischemia.
机标关键词：
分类号：R453(治疗学)R361(病理学)R741(神经病学)
资助基金：This study was supported by the National Natural Science Foundation of China,(No.8120093)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 265-271 )
英文信息