Predator stress-induced depression is associated with inhibition of hippocampal neurogenesis in adult male mice
Yan-Ping Wu
Hua-Ying Gao
Shu-Hua Ouyang
Hiroshi Kurihara
Rong-Rong He
Yi-Fang Li
摘要：Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis,which contribute to the development of depression.Few studies have investigated the effect of predator stress,a type of psychological stress,on depression and hippocampal neurogenesis in adult mice;we therefore investigated this in the present study.A total of 35 adult male Kunming mice were allocated to a cat stress group,cat odor stress group,cat stress + fluoxetine group,cat odor stress + fluoxetine group,or a control group (no stress/treatment).After 12 days of cat stress or cat odor stress,behavioral correlates of depression were measured using the open field test,elevated plus maze test,and dark-avoidance test.The concentrations of hippocampal 5-hydroxytryptamine and 5-hydroxyindoleacetic acid were measured using high-performance liquid chromatography-electrochemical detection.Neurogenesis was also analyzed using a bromodeoxyuridine and doublecortin double-immunostaining method.Cat stress and cat odor stress induced depression-like behaviors;this effect was stronger in the cat stress model.Furthermore,compared with the control group,cat stress mice exhibited lower 5-hydroxytryptamine concentrations,higher 5-hydroxyindoleacetic acid concentrations,and significantly fewer bromodeoxyuridine+/doublecortin+-labeled cells in the dentate gyrus,which was indicative of less neurogenesis.Tne changes observed in the cat stress group were not seen in the cat stress + fluoxetine group,which suggests that the effects of predator stress on depression and neurogenesis were reversed by fluoxetine.Taken together,our results indicate that depression-like behaviors induced by predator stress are associated with the inhibition of hippocampal neurogenesis.
机标关键词：
分类号：R459.9(治疗学)R364(病理学)
资助基金：This study was supported by the National Natural Science Foundation of China,(No.81473115)the Natural Science Foundation of Guangdong Province of China for Distinguished Young Scholars,(No.2017A030306004)the Youth Top-Notch Talent Support Program of Guangdong Province of China,(No.2016TQ03R586)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 298-305 )
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