Plasmid-based generation of neural cells from human fibroblasts using non-integrating episomal vectors
Shao-Bing Dai
Ting Shen
Ting-Ting Zheng
Jia-Li Pu
Xin-Zhong Chen
摘要：Differentiation of human fibroblasts into functional neurons depends on the introduction of viral-mediated transcription factors, which present risks of viral gene integration and tumorigenicity. In recent years, although some studies have been successful in directly inducing neurons through sustained expression of small molecule compounds, they have only been shown to be effective on mouse-derived cells. Thus, herein we delivered vectors containing Epstein-Barr virus-derived oriP/Epstein-Barr nuclear antigen 1 encoding the neuronal tran-scription factor, Ascl1, the neuron-specific microRNA, miR124, and a small hairpin directed against p53, into human fibroblasts. Cells were incubated in a neuron-inducing culture medium. Immunofluorescence staining was used to detect Tuj-1, microtubule-associated protein 2, neuron-specific nucleoprotein NeuN and nerve cell adhesion molecules in the induced cells. The proportion of Tuj1-positive cells was up to 36.7％ after induction for 11 days. From day 21, these induced neurons showed neuron-specific expression patterns of mi-crotubule-associated protein 2, NeuN and neural cell adhesion molecule. Our approach is a simple, plasmid-based process that enables direct reprogramming of human fibroblasts into neurons, and provides alternative avenues for disease modeling and neurodegenerative medicine.
机标关键词：
分类号：R456(治疗学)R318(医用一般科学)Q2(细胞生物学)
资助基金：This study was supported by the National Natural Science Foundation of China, (No. 81471126)the Natural Science Foundation of Zhejiang Province of China, (No. LY17H090005)a grant from the Medical Science and Technology Plan Project of Zhejiang Province of China, (No. 2016KYB119)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 501-505 )
英文信息