Cerebral organoids exhibit mature neurons and astrocytes and recapitulate electrophysiological activity of the human brain
Abraam M. Yakoub
摘要：Multiple protocols have been devised to generate cerebral organoids that recapitulate features of the devel-oping human brain, including the presence of a large, multi-layered, cortical-like neuronal zone. However, the central question is whether these organoids truly present mature, functional neurons and astrocytes, which may qualify the system for in-depth molecular neuroscience studies focused at neuronal and syn-aptic functions. Here, we demonstrate that cerebral organoids derived under optimal differentiation conditions exhibit mature, fully functional neurons and astrocytes, as validated by immunohistological, gene expression, and electrophysiological, analyses. Neurons in cerebral organoids showed gene expression profiles and electrophysiological properties similar to those reported for fetal human brain. These import-ant findings indicate that cerebral organoids recapitulate the developing human brain and may enhance use of cerebral organoids in modeling human brain development or investigating neural deficits that un-derlie neurodevelopmental and neuropsychiatric conditions, such as autism or intellectual disorders.
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论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 757-761 )
英文信息