Potassium bisperoxo (1,10-phenanthroline) oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases
Xiao-Li Tian
Shu-Yuan Jiang
Xiao-Lu Zhang
Jie Yang
Jun-He Cui
Xiao-Lei Liu
Ke-Rui Gong
Shao-Chun Yan
Chun-Yang Zhang
Guo Shao
摘要：Bisperoxo (1,10-phenanthroline) oxovanadate (BpV) can reportedly block the cell cycle. The present study examined whether BpV alters gene expression by affecting DNA methyltransferases (DNMTs), which would impact the cell cycle. Immortalized mouse hippocampal neu-ronal precursor cells (HT22) were treated with 0.3 or 3 μM BpV. Proliferation, morphology, and viability of HT22 cells were detected with an IncuCyte real-time video imaging system or inverted microscope and 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sul-fophenyl)-2H-tetrazolium, respectively. mRNA and protein expression of DNMTs and p21 in HT22 cells was detected by real-time polymerase chain reaction and immunoblotting, respectively. In addition, DNMT activity was measured with an enzyme-linked immuno-sorbent assay. Effects of BpV on the cell cycle were analyzed using flow cytometry. Results demonstrated that treatment with 0.3 μM BpV did not affect cell proliferation, morphology, or viability; however, treatment with 3 μM BpV decreased cell viability, increased expression of both DNMT3B mRNA and protein, and inhibited the proliferation of HT22 cells; and 3 μM BpV also blocked the cell cycle and increased expres-sion of the regulatory factor p21 by increasing DNMT expression in mouse hippocampal neurons.
机标关键词：
分类号：R453(治疗学)R364(病理学)
资助基金：This study was supported by the National Natural Science Foundation of China, (No. 81160244, 81360316, 81460283, 81660307)the Inner Mongolia Science Foundation of Chi-na, (No. 2018LH08078)the Baotou Health Foundation, China, (No. WSJJ2016008)the Inner Mon-golia Educational Research Foundation, China, (No. NJZY207)the Baotou Medical College Foundation, China of (No. BYJJ-DF201602, BYJJ-YF201615, BSJJ201617, BYJJ-QM201633, BYJJ-QM201656, BYJJ201502)the Science and Technology Planning Project of Baotou of China, (No. CX2017-5)the National Key R&D Pro-gram of China, (No. 2017YFC1308405 (to GS). The funding sources had no role in study conception and design, data analysis or interpretation, paper writing or deciding to submit this paper for publication)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 826-833 )
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