Acute histopathological responses and long-term behavioral outcomes in mice with graded controlled cortical impact injury
Si-Yi Xu
Min Liu
Yang Gao
Yang Cao
Jin-Gang Bao
Ying-Ying Lin
Yong Wang
Qi-Zhong Luo
Ji-Yao Jiang
Chun-Long Zhong
摘要：While animal models of controlled cortical impact often display short-term motor dysfunction after injury, histological examinations do not show severe cortical damage. Thus, this model requires further improvement. Mice were subjected to injury at three severities using a Pin-PointTM-controlled cortical impact device to establish secondary brain injury mouse models. Twenty-four hours after injury, hematoxylin-eosin staining, Fluoro-Jade B histofluorescence, and immunohistochemistry were performed for brain slices. Compared to the uninjured side, we observed differences of histopathological findings, neuronal degeneration, and glial cell number in the CA2 and CA3 regions of the hippocampus on the injured side. The Morris water maze task and beam-walking test verified long-term (14–28 days) spatial learning/memory and motor balance. To conclude, the histopathological responses were positively correlated with the degree of damage, as were the long-term behavioral manifestations after controlled cortical impact. All animal procedures were approved by the Institutional Animal Care and Use Committee at Shanghai Jiao Tong University School of Medicine.
机标关键词：
资助基金：This study was supported by the National Natural Science Foundation of China, (No. 81771332, 81571184, 81070990)the Shanghai Key Medical Discipline for Critical Care Medicine of China, (No. 2017zz02017)the Key Discipline Construction Project of Pudong Health Bureau of Shanghai of China, (No. PWZxk2017-23, PWYgf2018-05)the Outstanding Leaders Training Program of Pudong Health Bureau of Shanghai of China, (No. PWR12018-07)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 997-1003 )
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