Protective effects of organic extracts of Alpinia oxyphylla against hydrogen peroxide-induced cytotoxicity in PC12 cells
Li-Hong Duan
Meng Li
Chun-Bao Wang
Qing-Mei Wang
Quan-Quan Liu
Wan-Feng Shang
Ya-Jin Shen
Zhuo-Hua Lin
Tong-Yang Sun
Zheng-Zhi Wu
Ying-Hong Li
Yu-Long Wang
Xun Luo
摘要：Alpinia oxyphylla, a traditional herb, is widely used for its neuroprotective, antioxidant and memory-improving effects. However, the neu-roprotective mechanisms of action of its active ingredients are unclear. In this study, we investigated the neuroprotective effects of various organic extracts of Alpinia oxyphylla on PC12 cells exposed to hydrogen peroxide-induced oxidative injury in vitro. Alpinia oxyphylla was extracted three times with 95% ethanol (representing extracts 1–3). The third 95% ethanol extract was dried and resuspended in water, and then extracted successively with petroleum ether, ethyl acetate and n-butanol (representing extracts 4–6). The cell counting kit-8 assay and microscopy were used to evaluate cell viability and observe the morphology of PC12 cells. The protective effect of the three ethanol extracts (at tested concentrations of 50, 100 and 200 μg/mL) against cytotoxicity to PC12 cells increased in a concentration-dependent manner. The ethyl acetate, petroleum ether and n-butanol extracts (each tested at 100, 150 and 200 μg/mL) had neuroprotective effects as well. The op-timum effective concentration ranged from 50–200 μg/mL, and the protective effect of the ethyl acetate extract was comparatively robust. These results demonstrate that organic extracts of Alpinia oxyphylla protect PC12 cells against apoptosis induced by hydrogen peroxide. Our findings should help identify the bioactive neuroprotective components in Alpinia oxyphylla.
机标关键词：
分类号：R452(治疗学)R363(病理学)R364(病理学)
资助基金：This work was financially supported by the National Natural Science Foundation of China, (No. 81574038)the Natural Science Foundation of Guangdong Province of China, (No. 2017A030313842)the Science and Technology Foundation of Guangdong Province of China, (No. 2017A050506007)the Technology Research Foundation of Basic Research Project of Shenzhen City of China, (No. JCYJ20170412161254416)
论文发表日期：2020-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 682-689 )
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