A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis
Shuang-Xi Chen
Jia-Hui He
Yong-Jian Mi
Hui-Fan Shen
Melitta Schachner
Wei-Jiang Zhao
摘要：Oxidative stress contributes to the pathogenesis of neurodegenerative diseases. With the aim to find reagents that reduce oxidative stress, a phage display library was screened for peptides mimicking α2,6-sialyllactose (6′-SL), which is known to beneficially influence neural functions. Using Sambucus nigra lectin, which specifically binds to 6′-SL, we screened a phage display library and found a peptide com-prising identical sequences of 12 amino acids. Mimetic peptide, reverse peptide and scrambled peptide were tested for inhibition of 6′-SL binding to the lectin. Indeed, lectin binding to 6′-SL was inhibited by the most frequently identified mimetic peptide, but not by the reverse or scrambled peptides, showing that this peptide mimics 6′-SL. Functionally, mimetic peptide, but not the reverse or scrambled peptides, increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells, and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H2O2-induced oxidative stress. The combined results indicate that the 6′-SL mimetic peptide promotes neuronal survival and neuritogenesis, thus raising hopes for the treatment of neurode-generative diseases. This study was approved by the Medical Ethics Committee of Shantou University Medical College, China (approval No. SUMC 2014-004) on February 20, 2014.
机标关键词：
分类号：R453(治疗学)R741(神经病学)Q816(生物工程学(生物技术))
资助基金：the National Natural Science Foundation of China, (No. 81471279 and No. 81171138)Talent Support Grant from Shantou University Medical College, China, (No. 2501220118) This work was supported by the National Natural Science Foundation of China, No. 81471279 and No. 81171138(No. LD030302 (to MS). Financial support)a grant from the Li Kashing Foundation, (No. LD030302 (to MS). The funding bodies played no role in the study design, collection, analysis and interpretation of data, in the writing of the report, or in the decision to submit the paper for publication)
论文发表日期：2020-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1058-1065 )
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