Cerebral dopamine neurotrophic factor promotes the proliferation and differentiation of neural stem cells in hypoxic environments
Chao-Qun Lin1
Lu-Kui Chen2
1.School of Medicine,Southeast University,Nanjing,Jiangsu Province,China2.School of Medicine,Southeast University,Nanjing,Jiangsu Province,China;Department of Neurosurgery,Cancer Center,Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou,Guangdong Province,China
摘要：Previous research found that cerebral dopamine neurotrophic factor (CDNF) has a protective effect on brain dopaminergic neurons,and CDNF is regarded as a promising therapeutic agent for neurodegenerative diseases. However,the effects of CDNF on the proliferation,dif-ferentiation,and apoptosis of neural stem cells (NSCs),which are very sensitive to hypoxic environments,remain unknown. In this study,NSCs were extracted from the hippocampi of fetal rats and cultured with different concentrations of CDNF. The results showed that 200 nM CDNF was the optimal concentration for significantly increasing the viability of NSCs under non-hypoxic environmental conditions. Then,the cells were cultured with 200 nM CDNF under the hypoxic conditions of 90% N2,5% CO2,and 5% air for 6 hours. The results showed that CDNF significantly improved the viability of hypoxic NSCs and reduced apoptosis among hypoxic NSCs. The detection of markers showed that CDNF increased the differentiation of hypoxic NSCs into neurons and astrocytes. CDNF also reduced the expression level of Lin28 protein and increased the expression of Let-7 mRNA in NSCs,under hypoxic conditions. In conclusion,we determined that CDNF was able to reverse the adverse proliferation,differentiation,and apoptosis effects that normally affect NSCs in a hypoxic environ-ment. Furthermore,the Lin28/Let-7 pathway may be involved in this regulated function of CDNF. The present study was approved by the Laboratory Animal Centre of Southeast University,China (approval No. 20180924006) on September 24,2018.
机标关键词：
分类号：R446(诊断学)R364.4(病理学)R361+.3(病理学)
论文发表日期：2020-11-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 2057-2062 )
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