Neurons derived from human-induced pluripotent stem cells express mu and kappa opioid receptors
Zhi-Hai Ju
Xuan Liang
Yao-Yao Ren
Luo-Wa Shu
Yan-Hong Yan
Xu Cui
1.Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, China2.Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, China3.Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, China4.Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, China5.Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, China6.Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing, China
摘要：Neuroprotection studies have shown that induced pluripotent stem (iPS) cells have the possibility to transform neuroprotection research.In the present study, iPS cells were generated from human renal epithelial cells and were then differentiated into neurons. Cells in the iPS-cell group were maintained in stem cell medium. In contrast, cells in the iPS-neuron group were first maintained in neural induction medium and expansion medium containing ROCK inhibitors, and then cultivated in neuronal differentiation medium and neuronal maturation medium to induce the neural stem cells to differentiate into neurons. The expression of relevant markers was compared at different stages of differentiation. Immunofluorescence staining revealed that cells in the iPS-neuron group expressed the neural stem cell markers SOX1 and nestin on day 11 of induction, and neuronal markers TUBB3 and NeuN on day 21 of induction. Polymerase chain reaction results demonstrated that, compared with the iPS-cell group, TUBB3 gene expression in the iPS-neuron group was increased 15.6-fold. Further research revealed that, compared with the iPS-cell group, the gene expression and immunoreactivity of mu opioid receptor in the iPS-neuron group were significantly increased (38.3-fold and 5.7-fold, respectively), but those of kappa opioid receptor had only a slight change (1.33-fold and 1.57-fold increases, respectively). Together, these data indicate that human iPS cells can be induced into mu opioid receptor- and kappa opioid receptor-expressing neurons, and that they may be useful to simulate human opioid receptor function in vitro and explore the underlying mechanisms of human conditions.
机标关键词：
分类号：R459.9(治疗学)R363(病理学)R364(病理学)
论文发表日期：2021-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 653-658 )
英文信息