Bioinformatic analysis of cytokine expression in the proximal and distal nerve stumps after peripheral nerve injury
Xiao-Qing Cheng1
Wen-Jing Xu1
Xiao Ding1
Gong-Hai Han1
Shuai Wei1
Ping Liu1
Hao-Ye Meng1
Ai-Jia Shang2
Yu Wang3
Ai-Yuan Wang1
1.Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China;Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Chinese PLA General Hospital, Beijing, China;Key Lab of Musculoskeletal Trauma & War Injuries, Chinese PLA General Hospital, Beijing, China2.Department of Neurosurgery, Chinese PLA General Hospital, Beijing, China;Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China3.Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China;Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Chinese PLA General Hospital, Beijing, China;Key Lab of Musculoskeletal Trauma & War Injuries, Chinese PLA General Hospital, Beijing, China;Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
摘要：In our previous study, we investigated the dynamic expression of cytokines in the distal nerve stumps after peripheral nerve injury using microarray analysis, which can characterize the dynamic expression of proteins. In the present study, we used a rat model of right sciatic nerve transection to examine changes in the expression of cytokines at 1, 7, 14 and 28 days after injury using protein microarray analysis. Interleukins were increased in the distal nerve stumps at 1–14 days post nerve transection. However, growth factors and growth factor-related proteins were mainly upregulated in the proximal nerve stumps. The P-values of the inflammatory response, apoptotic response and cell-cell adhesion in the distal stumps were higher than those in the proximal nerve stumps, but the opposite was observed for angiogenesis. The number of cytokines related to axons in the distal stumps was greater than that in the proximal stumps, while the percentage of cytokines related to axons in the distal stumps was lower than that in the proximal nerve stumps. Visualization of the results revealed the specific expression patterns and differences in cytokines in and between the proximal and distal nerve stumps. Our findings offer potential therapeutic targets and should help advance the development of clinical treatments for peripheral nerve injury. Approval for animal use in this study was obtained from the Animal Ethics Committee of the Chinese PLA General Hospital on September 7, 2016 (approval No. 2016-x9-07).
机标关键词：
分类号：R446(诊断学)R741(神经病学)R318.04(医用一般科学)
论文发表日期：2021-05-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 878-884 )
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