Pannexin 1, a large-pore membrane channel,contributes to hypotonicity-induced ATP release in Schwann cells
Zhong-Ya Wei1
Hui-Lin Qu1
Yu-Juan Dai2
Qian Wang1
Zhuo-Min Ling2
Wen-Feng Su1
Ya-Yu Zhao1
Wei-Xing Shen2
Gang Chen3
1.Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China2.Medical School of Nantong University, Nantong, Jiangsu Province, China3.Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China;Medical School of Nantong University, Nantong, Jiangsu Province, China;Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China
摘要：Pannexin 1 (Panx 1), as a large-pore membrane channel, is highly permeable to ATP and other signaling molecules. Previous studies have demonstrated the expression of Panx 1 in the nervous system, including astrocytes, microglia, and neurons. However, the distribution and function of Panx 1 in the peripheral nervous system are not clear. Blocking the function of Panx 1 pharmacologically (carbenoxolone and probenecid) or with small interfering RNA targeting pannexins can greatly reduce hypotonicity-induced ATP release. Treatment of Schwann cells with a Ras homolog family member (Rho) GTPase inhibitor and small interfering RNA targeting Rho or cytoskeleton disrupting agents, such as nocodazole or cytochalasin D, revealed that hypotonicity-induced ATP release depended on intracellular RhoA and the cytoskeleton. These findings suggest that Panx 1 participates in ATP release in Schwann cells by regulating RhoA and the cytoskeleton arrangement. This study was approved by the Animal Ethics Committee of Nantong University, China (No. S20180806-002) on August 5, 2018.
机标关键词：
分类号：R446(诊断学)R741(神经病学)Q493.8(新陈代谢与营养)
论文发表日期：2021-05-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 899-904 )
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