Ascorbic acid accelerates Wallerian degeneration after peripheral nerve injury
Lixia Li
Yizhou Xu
Xianghai Wang
Jingmin Liu
Xiaofang Hu
Dandan Tan
Zhenlin Li
Jiasong Guo
摘要：Wallerian degeneration occurs after peripheral nerve injury and provides a beneficial microenvironment for nerve regeneration. Our previous study demonstrated that ascorbic acid promotes peripheral nerve regeneration, possibly through promoting Schwann cell proliferation and phagocytosis and enhancing macrophage proliferation, migration, and phagocytosis. Because Schwann cells and macrophages are the main cells involved in Wallerian degeneration, we speculated that ascorbic acid may accelerate this degenerative process. To test this hypothesis, 400 mg/kg ascorbic acid was administered intragastrically immediately after sciatic nerve transection, and 200 mg/kg ascorbic acid was then administered intragastrically every day. In addition, rat sciatic nerve explants were treated with 200 μM ascorbic acid. Ascorbic acid significantly accelerated the degradation of myelin basic protein-positive myelin and neurofilament 200-positive axons in both the transected nerves and nerve explants. Furthermore, ascorbic acid inhibited myelin-associated glycoprotein expression, increased c-Jun expression in Schwann cells, and increased both the number of macrophages and the amount of myelin fragments in the macrophages. These findings suggest that ascorbic acid accelerates Wallerian degeneration by accelerating the degeneration of axons and myelin in the injured nerve, promoting the dedifferentiation of Schwann cells, and enhancing macrophage recruitment and phagocytosis. The study was approved by the Southern Medical University Animal Care and Use Committee (approval No. SMU-L2015081) on October 15, 2015.
机标关键词：
分类号：R453(治疗学)R722.14+4(新生儿、早产儿疾病)Q564(维生素)
资助基金：the Program for Changjiang Scholars and Innovative Research Team in Universities of China,(No.IRT-16R37)the National Key Basic Research Program of China,(No.2014CB542202)the Science and Technology Project of Guangdong Province of China,(No.2015A020212024)Key Research&Development Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory of China,(No.2018GZR110104008)
论文发表日期：2021-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1078-1085 )
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