Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury
Poornima D.E.Weerasinghe-Mudiyanselage
Jeongtae Kim
Yuna Choi
Changjong Moon
Taekyun Shin
Meejung Ahn
摘要：Previous studies have shown that Ninjurin-1 participates in cell trafficking and axonal growth following central and peripheral nervous system neuroinflammation. But its precise roles in these processes and involvement in spinal cord injury pathophysiology remain unclear. Western blot assay revealed that Ninjurin-1 levels in rats with spinal cord injury exhibited an upregulation until day 4 post-injury and slightly decreased thereafter compared with sham controls. Immunohistochemistry analysis revealed that Ninjurin-1 immunoreactivity in rats with spinal cord injury sharply increased on days 1 and 4 post-injury and slightly decreased on days 7 and 21 post-injury compared with sham controls. Ninjurin-1 immunostaining was weak in vascular endothelial cells, ependymal cells, and some glial cells in sham controls while it was relatively strong in macrophages, microglia, and reactive astrocytes. These findings suggest that a variety of cells, including vascular endothelial cells, macrophages, and microglia, secrete Ninjurin-1 and they participate in the pathophysiology of compression-induced spinal cord injury. All experimental procedures were approved by the Care and Use of Laboratory Animals of Jeju National University (approval No. 2018-0029) on July 6, 2018.
机标关键词：
分类号：R459.9(治疗学)R364(病理学)R741(神经病学)
资助基金：MA was supported by the National Research Foundation of Korea(Grant No.NRF-2018R1D1A1B07050916)
论文发表日期：2021-07-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 1331-1335 )
英文信息